αv integrin  (Developmental Studies Hybridoma Bank)

Journal: Translational Neuroscience

Article Title: Thyroid hormone T3 induces Fyn modification and modulates palmitoyltransferase gene expression through αvβ3 integrin receptor in PC12 cells during hypoxia

doi: 10.1515/tnsci-2022-0347

Figure Lengend Snippet: JAK/STAT pathway’s protein phosphorylation in differentiated PC-12 cells in hypoxia. Densitometric analysis (arbitrary units of spot signal densities normalized to the positive control signals) showing the effect of 10 nM T3 and αvβ3-Ab integrin (µg/ml). Results represent the mean ± SEM of duplicate samples from two independent experiments. A significant level is defined as * p < 0.05 vs control and # p < 0.05 to T3.

Article Snippet: Differentiated cells were incubated with 10 nM T3 and αvβ3 blocking antibody (αvβ3-Ab, 1 μg/ml; sc-7312, Santa Cruz Biotechnology).

Techniques: Phospho-proteomics, Positive Control, Control

Journal: Translational Neuroscience

Article Title: Thyroid hormone T3 induces Fyn modification and modulates palmitoyltransferase gene expression through αvβ3 integrin receptor in PC12 cells during hypoxia

doi: 10.1515/tnsci-2022-0347

Figure Lengend Snippet: The action of T3 on the phosphorylation and palmitoylation of Fyn A. The ratio of palmitoylated and non-palmitoylated Fyn and p-Fyn forms. The palm-Fyn/Fyn ratio in differentiated PC-12 cells exposed to 10 nM T3 and µg/ml αvβ3-Ab integrin during 1 h of hypoxia. (a) Immunoblotting image of palm-Fyn and total Fyn; the palm-p-Fyn/p-Fyn ratio in differentiated PC-12 cells exposed to 10 nM T3 and αvβ3-Ab integrin (µg/ml) during 1 h of hypoxia. (b) Immunoblotting image of palm-p-Fyn and total p-Fyn; plot of the ratio of palm-p-Fyn to total p-Fyn. Plot of the ratio of palm-Fyn to total Fyn. Results represent the mean ± SEM of duplicate samples from two independent experiments. A significant level was defined as * p < 0.05 vs control and # p < 0.05 to T3.

Article Snippet: Differentiated cells were incubated with 10 nM T3 and αvβ3 blocking antibody (αvβ3-Ab, 1 μg/ml; sc-7312, Santa Cruz Biotechnology).

Techniques: Phospho-proteomics, Western Blot, Control

Journal: Translational Neuroscience

Article Title: Thyroid hormone T3 induces Fyn modification and modulates palmitoyltransferase gene expression through αvβ3 integrin receptor in PC12 cells during hypoxia

doi: 10.1515/tnsci-2022-0347

Figure Lengend Snippet: TH supplementation of hypoxic cells increases the proportion of phosphorylated to non-phosphorylated forms of palmitoylated Fyn kinase in differentiated PC-12 cells exposed to 10 nM T3 and αvβ3-Ab (µg/ml) integrin during 1 h hypoxia. Results represent the mean ± SEM of duplicate samples from two independent experiments. A significant level was defined as * p < 0.05. vs control and # p < 0.05. to T3.

Article Snippet: Differentiated cells were incubated with 10 nM T3 and αvβ3 blocking antibody (αvβ3-Ab, 1 μg/ml; sc-7312, Santa Cruz Biotechnology).

Techniques: Control

Journal: Translational Neuroscience

Article Title: Thyroid hormone T3 induces Fyn modification and modulates palmitoyltransferase gene expression through αvβ3 integrin receptor in PC12 cells during hypoxia

doi: 10.1515/tnsci-2022-0347

Figure Lengend Snippet: RT-qPCR analysis of genes ZDHHC2, ZDHHC3, ZDHHC8, ZDHHC9, and ZDHHC16 showing the effect of 10 nM T3 and αvβ3-Ab integrin (µg/ml) in differentiated PC-12 cells exposed to 1 h hypoxia

Article Snippet: Differentiated cells were incubated with 10 nM T3 and αvβ3 blocking antibody (αvβ3-Ab, 1 μg/ml; sc-7312, Santa Cruz Biotechnology).

Techniques: Control

Journal: Translational Neuroscience

Article Title: Thyroid hormone T3 induces Fyn modification and modulates palmitoyltransferase gene expression through αvβ3 integrin receptor in PC12 cells during hypoxia

doi: 10.1515/tnsci-2022-0347

Figure Lengend Snippet: The palmitoyltransferase gene expression levels of (a) ZDHHC2 and (b) ZDHHC9It were determined using the RT-PCR method in differentiated PC-12 cells exposed to 10 nM T3 and αvβ3-Ab integrin (µg/ml) during 1 h hypoxia. Results represent the mean ± SEM of duplicate samples from three independent experiments. A significant level was defined as * p < 0.05 vs control and # p < 0.05 to T3.

Article Snippet: Differentiated cells were incubated with 10 nM T3 and αvβ3 blocking antibody (αvβ3-Ab, 1 μg/ml; sc-7312, Santa Cruz Biotechnology).

Techniques: Gene Expression, Reverse Transcription Polymerase Chain Reaction, Control

Journal: Cell Journal (Yakhteh)

Article Title: 3,5,3'-Triiodo-L-Thyronine Regulates Actin Cytoskeleton Dynamic in The Differentiated PC-12 Cells during Hypoxia through An αvβ3 Integrin

doi: 10.22074/CELLJ.2022.557501.1059.

Figure Lengend Snippet: Immunoblotting analysis. A. Ratio of cofilin1/p-cofilin1 in the differentiated PC-12 cells with/without 10 nM T3 and αvβ3 antibody (αvβ3-Ab, 1 µg/ ml) during 1 hour hypoxia. Results of triplicate experiments are shown as mean ± SEM. Error-bars denote one standard error of the mean and asterisk and hash indicate a statistically significant difference. *; P=0.0045 vs. control and #; P=0.0058 vs. T3 treatment. B. Western blots of cofilin1 p-cofilin1, and beta-actin (loading control) in differentiated PC-12 cells with/without 10 nM T3 hormone and αvβ3 antibody (αvβ3-Ab, 1 µg/ml) during one-hour hypoxia. Bands were quantified by Image J.

Article Snippet: Differentiated PC-12 cells (5×10 6 cells per sample) were treated with T3 and αvβ3 integrin blocking antibody 1 μg/mL (23C6; sc-7312, Santa Cruz, USA), and incubated for 1 hour under hypoxic condition.

Techniques: Western Blot, Control

Journal: Cell Journal (Yakhteh)

Article Title: 3,5,3'-Triiodo-L-Thyronine Regulates Actin Cytoskeleton Dynamic in The Differentiated PC-12 Cells during Hypoxia through An αvβ3 Integrin

doi: 10.22074/CELLJ.2022.557501.1059.

Figure Lengend Snippet: Analysis of the cytotoxicity in differentiated PC-12 cells under hypoxia with/without 10 nM T3 hormone and αvβ3 antibody (αvβ3-Ab, 1 µg/ml) during 1 hour hypoxia by the LDH test as described in “Materials and Methods. Results of triplicate experiments are shown as mean ± SEM. Error-bars denote one standard error of the mean and asterisk and hash indicate a statistically significant difference. LDH; Lactate dehydrogenase, *; P=0.0050 vs. control and #; P=0.0153 vs. T3 hormone treatment.

Article Snippet: Differentiated PC-12 cells (5×10 6 cells per sample) were treated with T3 and αvβ3 integrin blocking antibody 1 μg/mL (23C6; sc-7312, Santa Cruz, USA), and incubated for 1 hour under hypoxic condition.

Techniques: Control

Journal: Cell Journal (Yakhteh)

Article Title: 3,5,3'-Triiodo-L-Thyronine Regulates Actin Cytoskeleton Dynamic in The Differentiated PC-12 Cells during Hypoxia through An αvβ3 Integrin

doi: 10.22074/CELLJ.2022.557501.1059.

Figure Lengend Snippet: Immunoblotting analysis. A. Diagram Analysis of the G- and F-actin content in differentiated PC-12 cells under hypoxia with/without 10 nM T3 hormone and αvβ3 antibody (αvβ3-Ab, 1 µg/ml) during 1 hour hypoxia. Results of triplicate experiments are shown as mean ± SEM. Error-bars denote one standard error of the mean and asterisk and hash indicate a statistically significant difference. *; P=0.0010 vs. control and #; P=0.0020 vs. T3 hormone treatment. B. Western blots of the G and F actins in the differentiated PC-12 cells with/without 10 nM T3 hormone treatment and αvβ3 antibody (αvβ3-Ab, 1 µg/ml) during 1 hour hypoxia. Bands were quantified by Image J.

Article Snippet: Differentiated PC-12 cells (5×10 6 cells per sample) were treated with T3 and αvβ3 integrin blocking antibody 1 μg/mL (23C6; sc-7312, Santa Cruz, USA), and incubated for 1 hour under hypoxic condition.

Techniques: Western Blot, Control

Journal: Cell Journal (Yakhteh)

Article Title: 3,5,3'-Triiodo-L-Thyronine Regulates Actin Cytoskeleton Dynamic in The Differentiated PC-12 Cells during Hypoxia through An αvβ3 Integrin

doi: 10.22074/CELLJ.2022.557501.1059.

Figure Lengend Snippet: Immunoblotting analysis. A. Diagram of the p-Fyn/Fyn ratio in differentiated PC-12 cells with/without 10 nM T3 and αvβ3 antibody (αvβ3-Ab, one µg/ml) during 1 hour hypoxia. Results of triplicate experiments are shown as mean ± SEM. Error-bars denote one standard error of the mean and asterisk and hash indicate a statistically significant difference. *; P=0.0010 vs. control and #; P=0.0138 vs. T3 treatment. B. Western blots of p-Fyn, Fyn and betaactin (loading control) in the differentiated PC-12 cells with/without 10 nM T3 hormone and αvβ3 antibody (αvβ3-Ab, 1 µg/ml) during one-hour hypoxia. Bands were quantified by Image J.

Article Snippet: Differentiated PC-12 cells (5×10 6 cells per sample) were treated with T3 and αvβ3 integrin blocking antibody 1 μg/mL (23C6; sc-7312, Santa Cruz, USA), and incubated for 1 hour under hypoxic condition.

Techniques: Western Blot, Control

Journal: Cell Journal (Yakhteh)

Article Title: 3,5,3'-Triiodo-L-Thyronine Regulates Actin Cytoskeleton Dynamic in The Differentiated PC-12 Cells during Hypoxia through An αvβ3 Integrin

doi: 10.22074/CELLJ.2022.557501.1059.

Figure Lengend Snippet: Analysis of the Rac 1 and NADPH oxidase activity in the differentiated PC-12 cells with/without 10 nM T3 hormone and αvβ3 antibody (αvβ3-Ab, 1 µg/ml) during 1 hour hypoxia. A. Level of Rac1 in the differentiated PC-12 cells with/without 10 nM T3 hormone and αvβ3 antibody (αvβ3-Ab,1 µg/ ml) during one h hypoxia. Results of triplicate experiments are shown as mean ± SEM. Error-bars denote one standard error of the mean and the asterisk and hash indicate a statistically significant difference *; P=0.0069 vs. control and #; P=0.0078 vs. T3 hormone treatment. B. NADPH oxidase activity in the differentiated PC-12 cells with/without 10 nM T3 hormone and αvβ3 antibody (αvβ3-Ab, 1 µg/ml) during 1 hour hypoxia. NADPH oxidase activity was measured as Relative Light Unit per minute (RLU/minute). Results of triplicate experiments are shown as mean ± SEM. Error-bars denote one standard error of the mean and asterisk or hash indicates a statistically significant difference. *; P=0.0010 vs. control and #; P=0.0014 vs. T3 hormone treatment.

Article Snippet: Differentiated PC-12 cells (5×10 6 cells per sample) were treated with T3 and αvβ3 integrin blocking antibody 1 μg/mL (23C6; sc-7312, Santa Cruz, USA), and incubated for 1 hour under hypoxic condition.

Techniques: Activity Assay, Control

Journal: Acta biomaterialia

Article Title: A robust vitronectin-derived peptide for the scalable long-term expansion and neuronal differentiation of human pluripotent stem cell (hPSC)-derived neural progenitor cells (hNPCs)

doi: 10.1016/j.actbio.2016.10.037

Figure Lengend Snippet: Flow cytometry of cell surface expression of various (A) integrin subunits and (B) CAMs of RiPSC-hNPCs cultured on LN, VDP, or Matrigel™. Gates were determined using isotype controls. Isotype controls used are listed in Supplementary Table 3.

Article Snippet: To assess for the effect of integrin αv blocking on hNPC adhesion to VDP, hNPCs were incubated in suspension with 5 μg/ml integrin αv blocking antibody (Millipore MAB1953Z) for 15 min at 37OC prior to plating onto VDP- or LN-coated substrates.

Techniques: Flow Cytometry, Expressing, Cell Culture

Journal: Acta biomaterialia

Article Title: A robust vitronectin-derived peptide for the scalable long-term expansion and neuronal differentiation of human pluripotent stem cell (hPSC)-derived neural progenitor cells (hNPCs)

doi: 10.1016/j.actbio.2016.10.037

Figure Lengend Snippet: (A) Phase contrast images of RiPSC-hNPCs treated with EDTA, integrin αv blocking antibody, heparin, and chondroitinase ABC prior to culture on LN- and VDP surfaces. (B) Cell counts were performed after 48 hours of culture. Data is presented as the mean ± S.E.M relative to untreated samples. All comparisons were made to untreated samples using Student’s t-test (*p< 0.05, **p < 0.01, ***p<0.001).

Article Snippet: To assess for the effect of integrin αv blocking on hNPC adhesion to VDP, hNPCs were incubated in suspension with 5 μg/ml integrin αv blocking antibody (Millipore MAB1953Z) for 15 min at 37OC prior to plating onto VDP- or LN-coated substrates.

Techniques: Blocking Assay